Original Article

Association of exosomal miR-34a with markers of dyslipidemia and endothelial dysfunction in children and adolescents with T1DM

10.4274/jcrpe.galenos.2020.2020.0134

  • Alshaymaa A. Ibrahim
  • Aliaa A. Wahby
  • Ingy Ashmawy
  • Rehan M. Saleh
  • Hend Soliman

Received Date: 21.06.2020 Accepted Date: 30.06.2020 J Clin Res Pediatr Endocrinol 0;0(0):0-0 [e-Pub] PMID: 32654473

Objective:

Dyslipidemia and endothelial dysfunction are common disorders and major causative factors for atherosclerosis in patients with T1DM. However, their pathophysiology in young patients with T1DM is still under evaluated. We aimed for the first time to assess the expression of exosomal miR-34a in serum of children and adolescents with T1DM and correlate this expression with markers of dyslipidemia and endothelial dysfunction.

Subjects and Methods:

The study included 120 T1DM patients and 100 control subjects. Assessment of exosomal miR-34a was done using qRT PCR. Measurement of Lipid profile was done using automated analyzer and serum endoglin and intracellular adhesion molecule (ICAM) levels were measured using ELISA technique.

Results:

Relative expression of miR-34a and serum endoglin and ICAM levels were higher in patients than controls and in in patients with dyslipidemia (42 patients) compared to patients without dyslipidemia (78 patients) (p=0.001, 0.01 respectively). Linear regression analysis revealed strong an independent association between exosomal miR-34a expression with those of total cholesterol, LDL, serum endoglin and serum ICAM after adjustment of other cofactors. The utility of miR-34a as indicator for associated dyslipidemia was tested using ROC curve which revealed AUC: 0.73 with CI:0.63-0.83 and p=0.001.

Conclusion:

This is the first study that reported the altered expression of exosomal miR-34a among children and adolescents with T1DM. Moreover, association of miR-34a with markers of dyslipidemia and endothelial dysfunction was identified, suggesting that it could play a role in regulation of lipid metabolism and endothelial function in T1DM.

Keywords: miR-34a, dyslipidemia, endothelial dysfunction, type 1 diabetes mellitus, endoglin, Intracellular adhesion molecule