Original Article

The Study of Serum Neuron-Specific Enolase and S100 calcium-binding protein B in Pediatric Diabetic Ketoacidosis


  • Hatem Hamed Elshorbagy
  • Naglaa Fathy Barseem
  • Akram Elshafey Elsadek
  • Ashraf Hamed Al-shokary
  • Yehia Hamed Abdel Maksoud
  • Sameh Elsayed Abdulsamea
  • Iman M. Talaat
  • Hany Abdelaziz Suliman
  • Naglaa M. Kamal
  • Waleed E. Abdelghani
  • Sanaa Mohammed Azab
  • Dalia Mohamed Nour El Din

Received Date: 01.12.2018 Accepted Date: 07.05.2019 J Clin Res Pediatr Endocrinol 0;0(0):0-0 [e-Pub] PMID: 31067852


Neuron-specific enolase(NSE) and S100 calcium-binding protein B (S100 B) are markers of different neurological disorders.


We aimed to investigate the relationship between brain injury markers (NSE and S-100 protein B) and severity of DKA in children.

Materials and Methods:

Eighty children with DKA, 40 with T1DM without DKA and 40 controls were enrolled. Serum NSE and S100 B. were measured in all participants. Among children with DKA, serum NSE and S100 B were measured at 3-time points of admission, 12hr, and 24 hr after starting treatment.


Children with DKA showed significantly higher serum levels of NSE at the 3-time points compared to children with T1DM without DKA and controls (p< 0.01) while serum S100 B levels were not different from controls and children with T1DM without DKA (P > 0.05). Patients with low Glasgow Coma Scale score(GCSS) and those with moderate and severe DKA had significantly higher levels of NSE at the 3-time points (P< 0.01for all) compared to patients with normal GCSS and those with mild DKA. No significant differences were found in serum S100 B levels according to the severity of DKA and GCS (P > 0.05). Younger age, lower GCSS, higher glucose and HA1c, lower pH, and bicarbonate serum levels were the risk factors associated with higher NSE in our patients.


Serum NSE is raised in patients with Type 1 DM and correlates with disease severity in patients with DKA. However, serum S100 B did not show any significant changes.

Keywords: Neuron, Specific Enolase, ketoacidosis, brain injury, S100 B, children