CTLA-4 (+49A/G) Polymorphism and Type-1 ?Diabetes in Turkish Children
Fatih Çelmeli1, Doğa Türkkahraman2, Deniz Özel3, Sema Akçurin4, Olcay Yeğin51Antalya Education and Research Hospital, Department of Pediatric Immunology, Antalya, Turkey2Antalya Education and Research Hospital, Department of Pediatric Endocrinology, Antalya, Turkey
3Akdeniz University Faculty of Medicine, Department of Medical Statistics, Antalya, Turkey
4Akdeniz University Hospital, Department of Pediatric Endocrinology, Antalya, Turkey
5Akdeniz University Hospital, Department of Pediatric Immunology, Antalya, Turkey
Objective: To evaluate the contribution of cytotoxic T-Iymphocyte antigen-4 (CTLA-4)+49A/G polymorphism to the susceptibility to type-1 diabetes (T1D) in Turkish children.
Methods: A case-control study was designed to include 91 Turkish children with T1D and 99 healthy controls. CTLA-4 (+99A/G) gene polymorphism typing was done by PCR amplification followed by restriction fragment length polymorphism method.
Results: The genotype and allele frequencies of the CTLA-4 (+99A/G) polymorphism in patients with T1D were not different from those in the controls (p>0.05). The allele frequency of G was 36.2% in patients with T1D, and 31.8% in controls (p>0.05). Additionally, this polymorphism was not associated with the clinical and laboratory characteristics of the patients with T1D (p>0.05).
Conclusions: Our case-control study suggests that the CTLA-4 (+99A/G) gene polymorphism is not associated with T1D in the Turkish population.
Manuscript Language: English
