ISSN: 1308-5727 | E-ISSN: 1308-5735
Volume : 7 Issue : 1 Year : 2024
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Turkish Society for Pediatric Endocrinology and Diabetes
Metabolic and Histopathological Effects of Fructose Intake During Pregestation, Gestation and Lactation in Rats and their Offspring [J Clin Res Pediatr Endocrinol]
J Clin Res Pediatr Endocrinol. 2015; 7(1): 19-26 | DOI: 10.4274/jcrpe.1776

Metabolic and Histopathological Effects of Fructose Intake During Pregestation, Gestation and Lactation in Rats and their Offspring

Erkan Sarı1, Ediz Yeşilkaya1, Ahmet Bolat1, Turgut Topal2, Bilal Altan3, Kürşat Fidancı1, Mehmet Saldır1, Galip Erdem1, Mustafa Gülgün1, Yasemin Gülcan Kurt4, Ahmet Güven3
1Gülhane Military Medical Academy, Department Of Pediatric Endocrinology, Ankara, Turkey
2Gülhane Military Medical Academy, Department Of Physiology, Ankara, Turkey
3Gülhane Military Medical Academy, Department Of Pediatric Surgery, Ankara, Turkey
4Gulhane Military Medical Academy, Department Of Biochemistry, Ankara, Turkey

Objective: Studies have demonstrated a significant relationship between maternal fructose intake and metabolic outcome in their offspring. However, there is a paucity of data about the long-term effects of fructose intake on the offspring of fructose-fed dams. Therefore, we planned a study to evaluate the long-term effects of fructose intake on the offspring of dam rats fed a high-fructose diet.
Methods: Sixteen virgin female Sprague-Dawley rats were divided into two groups. Group 1 received a regular diet and Group 2 a high-fructose diet. Both groups received their experimental diets for 8 weeks before conception. They were mated and continued to feed with their experimental diet during mating and during their pregnancy and lactation periods. After weaning, the offspring from each group were divided into two groups. Group 1A received a regular diet, Group 1B - a fructose diet, Group 2A - a regular diet and Group 2B received a fructose diet. After weaning, the offspring were anesthetized and blood samples were collected for biochemical analysis. Liver, kidney and retroperitoneal adipose tissue were harvested for histopathological examination. Primary antibodies against inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) were determined as early inflammation markers.
Results: After weaning, while daily water consumption was found to be significantly higher in Groups 2B and 1B (p<0.01), daily laboratory chow consumption was significantly lower in Groups 1A and 2A (p<0.01). Body weight was significantly higher in Groups 1B and 2B (p<0.01). Serum glucose, triglyceride, low-density lipoprotein cholesterol and very low-density lipoprotein cholesterol levels were found to be increased and high-density lipoprotein cholesterol levels decreased in Group 2B (p<0.05). The intensities of iNOS staining in the retroperitoneal adipose tissue, COX-2 staining in the liver and both iNOS and COX-2 staining in the kidney were higher in Group 2B (p<0.05).
Conclusion: Based on our findings, we believe that the offspring of dams which received a high fructose intake during their pregestation, gestation and lactation periods are at risk of developing metabolic syndrome in their later life only if they continue to receive a high intake of fructose. We therefore propose that the risk of developing metabolic syndrome can probably be reduced by modifying the diet of the offspring after weaning.

Keywords: Fructose,adipose,liver,kidney,metabolic effect,histopathological effect,rat offspring

Erkan Sarı, Ediz Yeşilkaya, Ahmet Bolat, Turgut Topal, Bilal Altan, Kürşat Fidancı, Mehmet Saldır, Galip Erdem, Mustafa Gülgün, Yasemin Gülcan Kurt, Ahmet Güven. Metabolic and Histopathological Effects of Fructose Intake During Pregestation, Gestation and Lactation in Rats and their Offspring. J Clin Res Pediatr Endocrinol. 2015; 7(1): 19-26
Manuscript Language: English
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