E-ISSN: 2791-8823
Volume : 14 Issue : 1 Year : 2022
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Abstracting & Indexing
Turkish Society for Pediatric Endocrinology and Diabetes
Genetic Indices Relationship to Hyperglycemia-associated Biomarkers: Consistency with miRNA Expression in Egyptian Children with T1DM [J Clin Res Pediatr Endocrinol]
J Clin Res Pediatr Endocrinol. 2022; 14(1): 76-86 | DOI: 10.4274/jcrpe.galenos.2021.2021.0080

Genetic Indices Relationship to Hyperglycemia-associated Biomarkers: Consistency with miRNA Expression in Egyptian Children with T1DM

Naglaa Fathy Barseem1, Marwa Mohamed Mahasab2, Ibrahem Fathy Zaed3, Aya Eldesoky A. Said3, Eman Masoud Abd El Gayed4
1Menoufia University Faculty of Medicine, Department of Pediatric, Unit of Genetic and Endocrinology, Shebein Elkom, Egypt
2Menoufia University Faculty of Medicine, Department of Family Medicine, Shebein Elkom, Egypt
3Menoufia University Faculty of Sciences, Department of Chemistry, Shebein Elkom, Egypt
4Menoufia University Faculty of Medicine, Department of Medical Biochemistry and Molecular Biology, Shebein Elkom, Egypt

INTRODUCTION: Micro RNAs (miRNAs) are gaining acceptance as novel biomarkers for the autoimmune disorders. However, miRNA profiles have not been investigated in individuals at risk of or diagnosed with type 1 diabetes mellitus (T1DM). To study the expression pattern of miRNAs in plasma obtained from patients with T1DM and compare with matched healthy controls.
METHODS: Equal numbers of patients with T1DM (90) and healthy-matched control children (90) were assessed for the expression profile of plasma miRNAs including miRNA-101-5p, miRNA-146-5p, miRNA-21-5p, miRNA-375, miRNA-126, and Let7a-5p using reverse transcriptase polymerase chain reaction methodology and quantitative real-time testing.
RESULTS: Analysis showed that miRNA-101, miRNA-21 and miRNA-375 were highly expressed, whereas, miRNA-146-5p, miRNA-126, and miRNA-Let7a-5p showed significantly low levels of expression in T1DM patients compared to controls (p<0.05). In addition, miRNA-101 and miRNA-146 correlated with age at diagnosis of T1DM and disease duration, respectively. Furthermore, multivariate analysis showed that miRNA-126 and Let7a-5p had a significant negative correlation with mean hemoglobin A1c (HbA1c) values.
DISCUSSION AND CONCLUSION: Dysregulation of the six miRNAs analyzed suggested a possible role as biomarkers in T1DM. miRNA-101 was correlated with age at diagnosis while miRNA-146 correlated with disease duration. Two further miRNAs correlated with the existing biomarker, HbA1c.

Keywords: Type 1 diabetes, miRNAs, plasma, q RT-PCR, gene expression

Naglaa Fathy Barseem, Marwa Mohamed Mahasab, Ibrahem Fathy Zaed, Aya Eldesoky A. Said, Eman Masoud Abd El Gayed. Genetic Indices Relationship to Hyperglycemia-associated Biomarkers: Consistency with miRNA Expression in Egyptian Children with T1DM. J Clin Res Pediatr Endocrinol. 2022; 14(1): 76-86

Corresponding Author: Naglaa Fathy Barseem, Egypt
Manuscript Language: English
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