ISSN: 1308-5727 | E-ISSN: 1308-5735
Volume : 13 Issue : 2 Year : 2023
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Abstracting & Indexing
Turkish Society for Pediatric Endocrinology and Diabetes
Methylation Status of GLP2R, LEP and IRS2 in Small for Gestational Age Children with and without Catch-up Growth [J Clin Res Pediatr Endocrinol]
J Clin Res Pediatr Endocrinol. 2021; 13(2): 136-145 | DOI: 10.4274/jcrpe.galenos.2020.2020.0070

Methylation Status of GLP2R, LEP and IRS2 in Small for Gestational Age Children with and without Catch-up Growth

Mario Angulo1, Diana Ramirez-Montaño2, Laura Torres-Canchala3, Ximena García4, Rodrigo Lemus4, Ana M. Aristizabal4, Danielle Floyd-Aristizábal4, Diana M. Dávalos4, Lorena Diaz-Ordoñez2, Harry Pachajoa5
1Fundación Valle del Lili, Pediatric Endocrinology Service, Colombia, South America
2Universidad Icesi, Centro de Investigaciones en Anomalías Congénitas y Enfermedades Raras, Colombia, South America
3Fundación Valle del Lili, Centro de Investigaciones Clínicas, Colombia, South America
4Universidad Icesi, Facultad de Ciencias de La Salud, Colombia, South America
5Universidad Icesi, Centro de Investigaciones en Anomalías Congénitas y Enfermedades Raras, Colombia, South America & Fundación Valle del Lili, Genetics Service, Colombia, South America

Objective: In small for gestational age (SGA) children, catch-up growth could be influenced by methylation of several genes involved in metabolism. Epigenetics may influence the development of metabolic diseases in adulthood. To compare the methylation of leptin (LEP), glucagon-like peptide-2 receptor (GLP2R), insulin receptor substrate-2 (IRS2) in SGA patients with and without catch-up growth.
Methods: Observational prospective study of SGA children. Demographical and clinical variables were collected from clinical records and parents’ questionnaire. Methylation status of LEP, IRS2, and GLP2R promoters was evaluated in DNA extracted from patient and one parent saliva samples.
Results: Forty-eight SGA patients were included. Twenty-six (54.2%) had catch-up growth phenotype and 22 (45.8%) did not. The median age was 5.2 years [RIC 4.1-6.8] without difference between groups (p=0.306). The catch-up group had increased appetite (42.3% vs 9.1%, p=0.008), family history of dyslipidemia (42.3% vs 27.3%) and diabetes (34.6% vs 22.7%) compared to non-catch-up group. Catch-up patients had significantly larger waist circumference compared to non-catch-up group (median 55 cm [RIC 52-58] versus median 49.5 cm [RIC46-52]; p<0.001). LEP and GLP2R were methylated in all samples. IRS2 was methylated in 60% of SGA patients without difference between groups (p=0.520).
Conclusion: There is no association between IRS2 methylation and catch-up growth among SGA patients. LEP and GLP2R were methylated in all SGA patients. Gene methylation may be implicated in metabolic disease later in life. More studies should be performed to confirm this hypothesis.

Keywords: Low birth weight, infant, small for gestational age, epigenetics, methylation, DNA, insulin resistance

Mario Angulo, Diana Ramirez-Montaño, Laura Torres-Canchala, Ximena García, Rodrigo Lemus, Ana M. Aristizabal, Danielle Floyd-Aristizábal, Diana M. Dávalos, Lorena Diaz-Ordoñez, Harry Pachajoa. Methylation Status of GLP2R, LEP and IRS2 in Small for Gestational Age Children with and without Catch-up Growth. J Clin Res Pediatr Endocrinol. 2021; 13(2): 136-145
Manuscript Language: English
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