ISSN: 1308-5727 | E-ISSN: 1308-5735
Volume : Issue : Year : 2022
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Turkish Society for Pediatric Endocrinology and Diabetes
Effect of Propolis on Precocious Puberty in Female Rats [J Clin Res Pediatr Endocrinol]
J Clin Res Pediatr Endocrinol. Ahead of Print: JCRPE-97658 | DOI: 10.4274/jcrpe.galenos.2022.2022-1-18

Effect of Propolis on Precocious Puberty in Female Rats

Recep Polat1, Erdem Çokluk2, Özcan Budak3, Fatıma Betül Tuncer4
1Sakarya University, Ministry of Health, Sakarya Training and Research Hospital, Department of Pediatric Endocrinology, Sakarya, Turkey
2Sakarya University Faculty of Medicine, Department of Medical Biochemistry, Sakarya, Turkey
3Sakarya University Faculty of Medicine, Department of Hıstology-Embryology, Sakarya, Turkey
4Sakarya University, Ministry of Health, Sakarya Training and Research Hospital, Department of Medical Biochemistry, Sakarya, Turkey

INTRODUCTION: Nutrition and exposure to various chemicals, including environmental pollution, insecticides, and plant phytoestrogens (having oestrogen-like effects), are environmental factors that affect puberty onset. We aimed to identify the effects of propolis on precocious puberty and the reproductive system in prepubertal female rats (ovary, endometrium, breast).
METHODS: Thirty-four 25-day-old prepubertal female Sprague-Dawley rats were included in the study. Rats were randomly divided into the propolis (n 17) and control groups (n 17). The primary endpoint was the number of rats that developed vaginal opening (It's a sign of puberty) at 12-day follow-up. In addition, the effect of propolis on ovary, uterus and breast tissue was evaluated.
RESULTS: Vaginal patency occurred earlier in the propolis group. At the same time, a greater number of rats developed vaginal opening. The number of ovarian follicles (in all follicles), endometrial thickness, and mammary gland secretory gland area were significantly higher in the propolis group than in the control group (p-values <0.001, <0.001, <0.001, respectively). In addition, Ki-67 activity in the endometrium, breast tissue and ovary was more intense in the propolis group compared to the control group (p-values <0.001, <0.001, <0.001, respectively).
DISCUSSION AND CONCLUSION: Propolis triggers precocious puberty in female rats, possibly by interacting with the oestrogen receptor. The mechanism of action of propolis should be considered before prescribing it. In addition, further studies are needed to explore the mechanism of action of propolis and to determine the component of propolis that triggers puberty.

Keywords: Phytoestrogens, Propolis, Precocious puberty, Rat



Corresponding Author: Recep Polat
Manuscript Language: English
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